Figure 3. rAAV2/5-α-syn mediated neurotoxicity in the SNpc (Experiment 2).

A, C. Surviving THir neurons in the α-syn overexpressing lesioned SNpc of 1.0 x 10¹³ vg/ml (A) and 1.0 x 10¹⁴ vg/ml (C) titer vector injected rats. B, D. THir neurons in the naïve, intact SNpc of 1.0 x 10¹³ (B) and 1.0 x 10¹⁴ (D) titer vector injected rats. E-F. α-syn overexpression resulted in significant THir neuron degeneration in both the 1.0 x 10¹⁴ and 1.0 x 10¹³ titer vector injected groups (*p ≤ 0.005) and magnitude of degeneration was not different between titer groups (F). G. Magnitude of THir neuron loss was equal across the SNpc. Percent survival of THir neurons in the SNpc of each of the eight sections demonstrates that degeneration occurred throughout the SNpc. Vector injection sites were -5.28 and -5.76 mm relative to bregma.