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. 2013 Nov 27;8(11):e81899. doi: 10.1371/journal.pone.0081899

Table 1. MS/MS identifications of differentially expressed proteins in Sb2000.1 compared to WT in SILAC experimentsa.

Systematic IDs     Putative protein name Unique peptides b (% seq. coverage)
  Ratio Sb2000.1/WT
 		   P-value
Error Factor
  		   TMDs c
  Fraction
Transport
LinJ.05.1140 vacuolar ATPase subunit-like protein 2 (5.88) 1,24 0,0583 1,26 0 M
LinJ.10.0400 FT1 folate/biopterin transporter, putative 3 (5.97) -2,22 0,0450 2,15 11 M
LinJ.10.0420 FT5 folate/biopterin transporter, putative 6 (6.99) -1,65 0,0618 1,76 11 M
LinJ.11.1050 pretranslocation protein, alpha subunit, putative, SEC61-like (pretranslocation process) protein, putative 2 (2.26) 1,46 0,0595 1,50 9 M
LinJ.23.0290 MRPA (ABCC3) ABC-thiol transporter 13 (9.37) 3,58 0,0002 1,76 8 M
LinJ.31.1240 vacuolar-type proton translocating pyrophosphatase 1, putative 9 (10.86) -1,36 0,0374 1,33 14 M
LinJ.32.2150 cop-coated vesicle membrane protein p24 precursor, putative,ER--golgi transport protein p24, putative 3 (24.38) 1,63 0,0267 1,42 1 M
LinJ.35.1840 COP-coated vesicle membrane protein gp25L precursor, putative,ER--golgi transport protein gp25L, putative 4 (18.69) 1,42 0,0160 1,25 2 M
Surface
LinJ.10.0500 GP63, leishmanolysin,metallo-peptidase, Clan MA(M), Family M8 23 (26.04) -1,91 1,257E-07 1,17 1 M
LinJ.30.0920 surface protein amastin, putative 5 (26.26) -1,73 0,0196 1,52 4 M
Cytoskeleton
LinJ.16.1550 kinesin, putative 4 (65.24) 2,75 0,0010 1,28 0 M
LinJ.23.0720 kinesin, putative 3 (3.51) 1,67 0,0013 1,19 0 M
Metabolism
LinJ.01.0510 long chain fatty acid CoA ligase, putative 4 (6.45) 1,47 0,0078 1,26 0 M
LinJ.06.1340 protoporphyrinogen oxidase-like protein 2 (14.29) -2,95 0,0084 1,74 1 M
LinJ.10.0310 isocitrate dehydrogenase [NADP], mitochondrial precursor, putative 3 (6.21) 1,27 0,0722 1,31 0 C
LinJ.11.1000 pyruvate phosphate dikinase, putative 2 (1.20) 1,38 0,0028 1,16 0 M
LinJ.11.1100 sterol 14-alpha-demethylase 6 (10.63) 1,45 0,0085 1,28 0 M
LinJ.13.0960 NADH-cytochrome B5 reductase, putative 6 (17.86) 1,47 0,0255 1,36 1 M
LinJ.15.1140 tryparedoxin peroxidase 12 (42.71) 1,38 0,0623 1,44 0 C
LinJ.21.0310 hexokinase, putative 2 (6.16) 1,40 0,0182 1,26 0 C
LinJ.21.0310 hexokinase, putative 10 (16.35) 1,53 0,0034 1,26 0 M
LinJ.24.0310 fumarate hydratase, putative 2 (4.55) 1,25 0,0766 1,31 0 C
LinJ.24.1700 succinate dehydrogenase flavoprotein, putative 2 (4.12) 1,20 0,0701 1,22 0 C
LinJ.24.1700 succinate dehydrogenase flavoprotein, putative 8 (10.05) 1,37 0,0771 1,44 1 M
LinJ.30.2590 formate--tetrahydrofolate ligase 1 (2.25) 1,57 0,0408 1,50 0 C
LinJ.30.2920 aldehyde dehydrogenase, putative 3 (3.59) 1,55 0,0089 1,25 0 M
LinJ.30.3000 glyceraldehyde 3-phosphate dehydrogenase, glycosomal 2 (3.88) 1,39 0,0778 1,47 0 M
LinJ.32.3510 GCVL-2 dihydrolipoamide dehydrogenase, putative 5 (5.25) 1,23 0,0017 1,11 0 M
LinJ.36.3630 2-oxoglutarate dehydrogenase E1 component, putative 3 (3.56) 1,38 0,0840 1,46 0 M
LinJ.36.6960 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 10 (21.16) 1,26 0,0471 1,25 0 C
Protein folding
LinJ.27.2350 heat shock protein DNAJ, putative 5 (16.92) -1,25 0,0708 1,28 0 C
LinJ.28.3060 heat-shock protein hsp70, putative 5 (6.79) 1,43 0,0018 1,17 0 M
LinJ.30.2540 heat shock 70-related protein 1, mitochondrial precursor, putative 3 (4.85) 1,25 0,0373 1,23 0 M
LinJ.36.2140 chaperonin Hsp60, mitochondrial precursor 40 (52.49) 1,32 0,0008 1,14 0 C
Proteolysis
LinJ.14.0920 calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 2 (17.39) -1,44 0,0570 1,48 0 C
LinJ.20.1320 calpain-like cysteine peptidase, putative,calpain-like cysteine peptidase, Clan CA, family C2 6 (52.32) -2,55 0,0023 1,64 0 M
LinJ.27.0500 calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 2 (6.17) 1,72 0,0060 1,28 0 M
LinJ.36.1730 proteasome beta 5 subunit, putative 3 (11.92) 1,31 0,0345 1,27 0 C
Transcription, Translation
LinJ.17.0110 elongation factor 1-alpha 11 (21.87) 1,26 0,0003 1,11 0 M
LinJ.29.1920 40S ribosomal protein S15A, putative 7 (25.38) 1,24 0,0576 1,25 0 M
LinJ.29.2580 60S ribosomal protein L13, putative 4 (12.27) -1,26 0,0515 1,26 0 M
LinJ.30.3650 40S ribosomal protein S14 4 (20.14) -1,59 0,0021 1,20 0 C
LinJ.36.0020 histone H4 6 (34.00) 1,41 0,0569 1,43 0 M
Others
LinJ.20.1350 SMP-1 small myristoylated protein-1, putative 10 (19.85) 1,28 0,0941 1,42 0 M
LinJ.28.2430 glycosomal membrane protein, putative 3 (11.71) 1,62 0,0007 1,20 0 M
LinJ.36.1420 Transitional endoplasmic reticulum ATPase, putative,valosin-containing protein homolog 8 (14.78) -1,26 0,0774 1,30 0 C
Hypothetical
LinJ.06.0640 hypothetical protein, conserved 4 (5.05) -1,29 0,0242 1,22 0 C
LinJ.08.1010 hypothetical protein, conserved 4 (3.01) 1,34 0,0688 1,42 0 M
LinJ.14.0460 hypothetical protein, conserved 2 (12.11) -1,40 0,0808 1,51 0 C
LinJ.17.0010 hypothetical protein, conserved 8 (7.62) 1,26 0,0252 1,21 0 M
LinJ.24.2250 hypothetical protein, conserved 3 (7.48) 1,47 0,0123 1,25 0 M
LinJ.26.1020 hypothetical protein, conserved 3 (4.19) 1,32 0,0125 1,19 1 M
LinJ.26.1960 hypothetical protein, conserved 4 (4.48) 1,29 0,0030 1,15 0 M
LinJ.27.0720 hypothetical protein, conserved 3 (2.92) -1,94 0,0969 3,62 0 C
LinJ.29.1600 hypothetical protein, conserved 3 (5.06) -2,69 0,0004 1,28 14 M
LinJ.30.3190 hypothetical protein, conserved 6 (15.57) 1,26 0,0554 1,27 1 M
LinJ.34.3960 hypothetical protein, conserved 4 (3.68) 1,24 0,0616 1,26 1 M
LinJ.35.0140 hypothetical protein, conserved 3 (17.65) 1,51 0,0758 1,68 1 M
LinJ.35.1010 hypothetical protein, conserved 2 (3.71) -2,11 0,0992 2,98 0 C
LinJ.36.5310 hypothetical protein, conserved 1 (1.44) -1,37 0,0491 1,36 0 C

aTMDs, transmembrane domains; M, membrane-enriched fraction; C, cytosolic fraction. bPeptide identifications were accepted if they reached greater then 95% probability as specified by the Peptide Prophet algorithm. cTMDs were predicted using TMHMM v2.0.