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. 2013 Nov 27;8(11):e81903. doi: 10.1371/journal.pone.0081903

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© 2013 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cav-1^+/+ and cav-1^-/- MLEC were serum starved for 4 h then incubated with Alexa488-BSA (50 µg/ml) in HBSS. Fluorescence signals were recorded from 0 min to 30 min under total internal reflection fluorescence (TIRF) microscopy. Images from indicated time points (1, 5, 10, 15 and 20 min) are shown. (B) Quantitative analyses of Alexa488-BSA uptake in cav-1^+/+ and cav-1^-/- MLEC were performed with MetaMorph software by measuring the fold change of fluorescence intensity compared to cav-1^+/+ MLEC at 1 min after treatment.