Figure 2. The genome tree based on ANI values showing the evolutionary relationships among 53 Mycobacterium abscessus genomes available in NCBI microbial genome resources (http://www.ncbi.nlm.nih.gov/genome).

To convert the ANI into a distance, its complement to 1 was taken. From this pairwise distance matrix, an ANI tree was constructed using the UPGMA clustering method.