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. 2013 Nov 27;8(11):e81781. doi: 10.1371/journal.pone.0081781

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© 2013 Han et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cultured in growth medium, MSCs in passage (P) 10 were larger and fatter compared to cells in P1 (A & B). Real-Time PCR analysis showed expressional changes of genes involved in stem cell pluripotency and osteogenic differentiation (C). The experiment was repeated three time with similar results, and representative results from one experiment were shown (*P<0.01). ALP, asalkaline phosphatase; OPN, osteopontin.