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. 2013 Nov 27;8(11):e81781. doi: 10.1371/journal.pone.0081781

Figure 5. Influence of TSA on the expression and histone H3 acetylation of pluripotent gene in hMSCs.

Figure 5

Passage (P)1 hMSCs were grown in the presence of TSA (at 6.25 nM) or vehicle DMSO to passage 6. (A) Real-Time PCR analysis of Oct4, TERT, Sox2, Nanog, REX1 and CD133 genes of the cells in P1 and P6 (A, n=3, *P<0.01 when P6-DMSO compared to P1 and to P6-TSA). (B) Acetylation of histone H3 in K9 and K14 in Oct4, Sox2 and TERT genes in DMSO- or TSA-treated hMSCs (*P<0.01 when P6-DMSO compared to P1 and to P6-TSA). The experiments were repeated three times with similar results.