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. 2013 Nov 27;8(11):e81266. doi: 10.1371/journal.pone.0081266

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© 2013 CORDELIERES et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cells were imaged every four minutes for 12 hours and experiments were repeated three times. All cells that were lost during the automatic tracking were re-analyzed by the manual method. We compared the following variables for the population of cells followed by the software (41 cells) and the population of cells lost by the software (26 cells), both populations being analyzed manually here.

A. Total distance of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5756).

B and C. Average distance of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5757 and p = 0.5698 for the average distance and standard deviation, respectively).

D. Maximum distance of WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.4433).

E. Persistence of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5632).

F. Pause of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.6459).

G and H. Average migration speed of WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5953 and p = 0.5057 for the average speed and standard deviation, respectively).

I. Average acceleration of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.6894).