Table 1. Primers and probes used for pathogen confirmation or prevalence estimation in ticks.

Pathogen	Target	Primers and Probe Sequence (5′ → 3′)	Amplicon size (bp)	Reference
Borrelia miyamotoi	glpQ	CACGACCCAGAAATTGACACA	94	This study
		GTGTGAAGTCAGTGGCGTAAT
		TCGTCCGTTTTCTCTAGCTCGATTGGG
Coxiella burnetti	idc	AGGCCCGTCCGTTATTTTACG	74	Adapted from [47]
		CGGAAAATCACCATATTCACCTT
		TTCAGGCGTTTTGACCGGGCTTGGC
Ehrlichia canis	dsb	AATACTTGGTGAGTCTTCACTCA	110	Adapted from [30]
		GTTGCTTGTAATGTAGTGCTGC
		AAGTTGCCCAAGCAGCACTAGCTGTAC
Francisella tularensis	tul4	ACCCACAAGGAAGTGTAAGATTA	76	Adapted from [48]
		GTAATTGGGAAGCTTGTATCATG
		AATGGCAGGCTCCAGAAGGTTCTAAGT
Rickettsia felis	orfB	CCCTTTTCGTAACGCTTTGCT	149	49
		GGGCTAAACCAGGGAAACCT
		TGTTCCGGTTTTAACGGCAGATACCCA
Rickettsia canadensis	ompA	AGTGGGACATTGGGTGTTGC	129	This study
		GGCAACACCTTGAGCATTATC
		CTCCTGCCTGCGTTATACCATGCCA
Candidatus Neoehrlichia mikurensis	groEL	AGAGACATCATTCGCATTTTGGA	96	This study
		TTCCGGTGTACCATAAGGCTT
		AGATGCTGTTGGATGTACTGCTGGACC