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. 2013 Nov 27;8(11):e81309. doi: 10.1371/journal.pone.0081309

Figure 6. Effect of the IC2B peptide on pulmonary artery Akt and ERK signaling.

Figure 6

Rats were treated with hypoxia and injected with IC2B peptide for 24 h. Pulmonary artery rings were isolated and pre-incubated with or without 1uM BQ123 for 30 mins. Then, the rings were treated with or without 10 nM ET-1 for 20 min. Protein lysates were probed for pAkt, pERK, tAkt and tERK. Western blot analysis comparing basal pAkt and pERK levels in pulmonary artery rings from normoxic and hypoxic rats (A). Western blot analysis of rat pulmonary artery rings from hypoxic rats injected with the IC2B peptide (B). Bar graphs illustrate the densitometric ratios of pAkt (C) and pERK (D) normalized to tAkt and tERK, respectively. Blots are representative of triplicate experiments. Data are means + SEM N=3 * P<0.05 vs hypoxic control. # P<0.05 vs hypoxic ET-1 plus BQ123. a.u=arbitrary unit.