Figure 2.

Phenotype and expression of TNF by Th subsets after transfer into Rag1^−/− mice. In vitro differentiated Th subsets were co-transferred with freshly flow-sorted Tregs at 1:1 ratio into Rag1^−/− mice. After 5 weeks, Expression of IFNγ, IL-13, IL-17, Foxp3, and TNF by Th subsets recovered from spleen of recipient mice was analyzed by FACS, gating on CD4⁺CD45.2⁻ cells. Representative FACS data from 3 separate experiments are shown.