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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cytokine. 2013 Sep 27;65(1):10.1016/j.cyto.2013.09.008. doi: 10.1016/j.cyto.2013.09.008

Figure 4.

Figure 4

Effect of neutralizing anti IL-2 Ab and inhibitor of TNF on Tregs co-transferred with Th17 cells. Flow-sorted Tregs (CD45.2+) were co-transferred with Th17 cells (CD45.2−) into Rag1–/– mice as described in Fig 2. The mice were i.p. treated with anti-IL2 Ab or/and TNFR-Fc fusion protein (100 µg) once a week. After 5 wks, the proportion of Tregs in transferred T cells present in the spleen was analyzed by FACS. Typical FACS plots are shown in (A) and summary (N=12) is shown in (B). The number of transferred Treg cells in spleen is shown in (C, N=12), in LNs (mesenteric region) is shown in (E, N=6) and number of Foxp3+ Tregs in the spleen is shown in (D, N=12). Data in summary are pooled from 3 separate experiments. Comparison between indicated groups: *p < 0.05; **p < 0.01.