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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cytokine. 2013 Sep 27;65(1):10.1016/j.cyto.2013.09.008. doi: 10.1016/j.cyto.2013.09.008

Figure 5.

Figure 5

TNF-TNFR2 pathway is crucial for the stimulation of Th17 on Tregs. Flow-sorted Tregs (CD45.2+) from WT mice or from TNFR2–/– mice were transferred into Rag1–/– mice, with or without Th17 cells (CD45.2−). After 35 days, the expression of Foxp3 by transferred Tregs was analyzed by FACS, gating on live TCRβ+CD45.2+ cells. (A) Shows typical flow plots and (B) shows summary (N=3). (C) The absolute number of transferred Treg cells (C, N=3) and (D) absolute number of Foxp3+ Tregs in the spleen (N=3). Data shown are representatives of 2 separate experiments with same results. Comparison between indicated groups: *p < 0.05; **p < 0.01; *** p < 0.001.