Figure 2. ASPP2 is induced upon anthracycline exposure to promote apoptosis.

(A) Intracellular ASPP2 protein expression levels increase upon daunorubicin treatment in a dose-dependent manner in the acute myeloid leukemia HL60 cell line and the T-lymphoblastic leukemia Jurkat cell line. A flow cytometry based assay is shown (A-1) which is confirmed by a Western immunoblot (A-2). (B) Lipofection of Jurkat and HL60 leukemia cell lines with specific ASPP2 siRNA or random siRNA as negative controls was performed. Cells were treated with daunorubicin (5 nM) for 48 hours and induction of apoptosis was measured using an Annexin V-based assay and flow cytometry.