Skip to main content
. 2013 Nov 27;8(11):e81768. doi: 10.1371/journal.pone.0081768

Figure 4. J100D-produced mIL-15/IL-15Rα stimulates survival and proliferation of enriched NK cells.

Figure 4

Enriched NK cells were stained with CFSE and cultured in the presence of supernatant obtained from cells either mock infected or infected with the indicated viruses. Recombinant mIL-15/IL-15Rα complex was added to mock supernatant at 10ng/mL for the positive control, and the J100D sample was diluted to contain a final mIL-15/IL-15Rα complex concentration of 10ng/mL. Cells were cultured for 7 days prior to analysis. A) Forward vs. side scatter plots indicating survival of a population when mIL-15/IL-15Rα is present in the culture media. B - C) NKp46 staining and proliferation (as indicated by CFSE dilution) of cells present within the gate shown in A. Histograms presented in panel C are derived from the box gates in panel B. Data is representative of three independent experiments.