Table 2.
Primer sequences
Universal qPCR | Forward | Uni334F | 5’- ACTCCTACGGGAGGCAGCAGT-3’ |
Universal qPCR | Reverse | Uni514R | 5’- ATTACCGCGGCTGCTGGC-3’ |
Bifidobacteria qPCR | Forward | Bif F | 5’- GCGTGCTTAACACATGCAAGTC-3’ |
Bifidobacteria qPCR | Reverse | Bif R | 5’- CACCCGTTTCCAGGAGCTATT-3’ |
Taqman probe | Bif P | 5’- [6-FAM]TCACGCATTACTCACCCGTTCGCC[BHQ1]-3’ | |
PCR for TRFLP | Forward | Uni331F-FAM | 5’-[5FAM]-TCCTACGGGAGGCAGCAGT-3’ |
PCR for TRFLP | Reverse | 1492R | 5’-GGTTACCTTGTTACGACTT-3’ |
PCR for Bac-TRFLP | Forward | NLAB2F | 5’[5HEX]-GGCGGCGTGCCTAATACATGCAAGT-3’ |
PCR for Bac-TRFLP | Reverse | WLAB1R | 5’-TCGCTTTACGCCCAATAAATCCGGA-3’ |
PCR for Bif-TRFLP | Forward | NBIF389 | 5' [HEX]-GCCTTCGGGTTGTAAAC-3' |
PCR for Bif-TRFLP | Reverse | NBIF1018REV | 5'-GACCATGCACCACCTGTG-3' |