Figure 8.

Cyclin D1 rescued the proliferation defect induced by Dyrk1A overexpression in vivo. E14.5 embryos were coelectroporated with pCAG–cyclin D1–HA and pCAG–nEGFP or pCAG–Dyrk1AEGFP and pulsed by BrdU injection. At 24 h after IUE, coronal sections of the lateral neocortex were subjected to immunofluorescence staining against Tuj1 (A) or BrdU (C). A, Representative confocal images showing the localization of transfected cells (green) and Tuj1 immunofluorescence (red). B, Quantification of the data obtained as in A. The localization of cells expressing Dyrk1A in different zones of the neocortex was comparable with that of control nEGFP-expressing cells. C, BrdU labeling of embryos coelectroporated in utero with cyclin D1 and nEGFP or Dyrk1A plasmids. Higher magnifications of the boxed areas are shown above each overlay image to illustrate representative patterns of colocalization. The percentage of cells expressing Dyrk1A that was positive for BrdU did not differ significantly when compared with control nEGFP⁺ cells. Scale bars: A, 50 μm; C, 100 μm. Bar graphs show SEM.