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. 2010 Aug 25;30(34):11414–11425. doi: 10.1523/JNEUROSCI.2127-10.2010

Figure 2.

Figure 2.

Aβ (1 μm) affects mEPSC amplitude and depresses AMPA-mediated synaptic transmission. A, mEPSCs recorded in the presence of TTX (1 μm) and bicuculline (3 μm); examples of traces recorded before, during, and after perfusion with 1 μm Aβ(1-42). Calibration: horizontal, 10 s; vertical, 20 pA. B, The plot represents average mEPSC amplitude recorded in control condition (vehicle), during 10 min perfusion with 1 μm Aβ, and after Aβ washout. mEPSC amplitude was significantly decreased (*p < 0.05) during Aβ perfusion; after washing out Aβ, the amplitude of mEPSCs returned to control values. C, Ten minute perfusion with 1 μm Aβ had no effect on the frequency of mEPSC; plot represents average frequency of mEPSCs recorded in control condition (vehicle), during 1 μm Aβ perfusion, and after Aβ washout. D, Traces represent examples of evoked EPSCs that were recorded at −70 and +50 mV, respectively, in control (vehicle) and after 1 μm Aβ perfusion. The amplitude of AMPA EPSC component was measured at −70 mV, whereas NMDA EPSC component was calculated at +50 mV, 50 ms after the AMPA peak (see dashed lines). The gray traces represent recordings at +50 mV, where the AMPA component was isolated using AP5 (50 μm). Calibration: horizontal, 50 ms; vertical, 50 pA. E, Perfusion with 1 μm Aβ caused a significant reduction of AMPA-mediated EPSC peak amplitude; in the top panel, the plot represents averaged relative amplitude of AMPA EPSCs recorded at −70 mV in control condition (vehicle), during 10 min of 1 μm Aβ perfusion, and after Aβ washout (*p < 0.01). In the bottom panel, a complete I–V curve is reported for AMPA EPSCs recorded in control (vehicle), during Aβ perfusion. F, Aβ did not significantly affect NMDA currents; in the top panel, the plot represents averaged relative amplitude of NMDA EPSCs recorded at +50 mV in control condition (vehicle), during 10 min of 1 μm Aβ perfusion, and after Aβ washout (*p < 0.01). In the bottom panel, a complete I–V curve is reported for NMDA EPSCs recorded in control (vehicle) conditions and under Aβ perfusion. Error bars indicate SEM.