Figure 11.

Chronic exposure of organotypic slice cultures to PGE2 or TTX induced increased apoptosis. A, Examples of staining for active caspase-3 in cortical layers revealed increased cell death in PGE2- and TTX-treated or TTX+PGE2-treated organotypic slice cultures. B, Quantification of caspase-3-positive cells/mm² showed a significantly higher number of cells in TTX- and TTX+PGE-treated cultures than in control (**p < 0.01, ***p < 0.001) and significantly higher amount of labeled cells in the TTX+PGE2-treated cultures than in the cultures treated with one drug (^#p < 0.05, ^###p < 0.001, ^‡p < 0.05).