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Representative fluorescence microscopy images of SAOS-2 cells either fixed with 4% PFA or incubated with 0.2 M trehalose, 2 mM calcein, with or without PP-50 (200 μg/ml), at pH 7.05. The cells were subsequently stained with Hoechst 33342 (2 μg/ml) and PI (2 μg/ml) for 15 min, following three washes with DPBS. The scale bars represent 25 μm.
