Figure 2. Analysis of humoral responses, (A) total IgG, (B) IgG1 and (C) IgG2a antibody levels in mice immunized with HCVcp in different formulations by ELISA. In control groups HCVcp is replaced by Normal Saline. Each formulation is written on the horizontal axis of diagrams (see text for detailed procedure). Data shows antibody titers in three independent experiments. Data are expressed as means ± standard errors per group and “*” (star labeled figure) indicates the significant differences (P < 0.05) according to the Mann–Whitney non-parametric test.