Figure 3. Type and Magnitude of Cellular Responses Induced in HCVcp-Adjuvanted Immunized Mice, (A) Enzyme-linked-immunospots assay for detection of class I-binding C39 peptide specific IFN-γ and IL-4-releasing T cells; results are shown as the numbers of spot-forming-cells (SFCs) per 106 splenocytes (3 weeks after the last immunization in BALB/c mice). Mice were immunized in two different regimens with HCVcp either formulated in BCG or F127 as adjuvant. Results indicated the significant differences between different regimens of immunization and a higher degree of IFN-γ and IL-4-releasing T cells in case of HCVcp+BCG and HCVcp+F127 formulations, respectively. (B) Assessment of mice spleen T lymphocyte proliferation using the BrdU ELISA; Proliferation assay of BCG-core and F127-core vaccinated mice splenocyts and control groups showed significant difference of stimulation index (SI) in favor of BCG formulation. (C) Cytotoxic T-lymphocyte (CTL) assays; mice immunized with HCVcp+F127 showed significantly higher specific lysis than mice immunized with HCVcp+BCG. Statistical analysis was done by the Mann-Whitney non-parametric test and ANOVA analyses.“*”(star labeled figure) indicates the significant differences (P < 0.05).