Fig. 4.

IgE is regulated by intraepidermal γδ T cells and is dependent on NKG2D. (A and B) Total and ova-specific serum Ig induction (A) and IL-13 secretion in the skin draining LN (B) of (orange) TcrgV5^−/−TcrdV1^−/− relative to (black) WT mice after epicutaneous immunization with ova, as described in Fig. 1. (C) Serum ova-specific Ig and total IgE responses after epicutaneous immunization with ova, as described in Fig. 1, in (black) WT and (green) Klrk1^−/− mice as measured with ELISA. (D) ova-dependent IL-13 production in skin draining LNs from (green) Klrk1^−/− and (black) WT mice, after epicutaneous immunization and 72-hour in vitro restimulation with ova, determined with ELISA. Data are expressed as mean ± 1 SEM, and n = 6 to 10 mice per group. All experiments [(A) to (D)] were repeated twice with comparable results. (E) Total serum IgE response in (black) WT C57BL/6 and (blue) Myd88^−/− mice 10 days after primary epicutaneous immunization, as described in Fig. 1, or in unimmunized mice. Data are expressed as mean ± 1 SEM, and n = 4 unimmunized mice and n = 7 immunized mice per group.