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. 2013 Nov 28;3:3365. doi: 10.1038/srep03365

Figure 5. EBOV GP-specific cell mediated immune response during rechallenge.

Figure 5

The cell mediated immune response was examined on days 7, 14 and 28 of the rechallenge utilizing (A) the IFN-γ ELISPOT assay, and the (B) CD8+ and (C) CD4+ flow cytometric intracellular cytokine staining (ICS) assay. PBMCs were added to 3 different peptide pools spanning the entire EBOV GP region, or to media alone. For flow cytometry, a response was considered positive if it was at least twice the media only background, in which case the background value was subtracted. The graphs represent the sum of the three pools.