Figure 6. CD4+ and CD8+ EBOV GP-specific memory T cell response prior to rechallenge.

Flow cytometry was utilized to determine the EBOV GP-specific T cell memory immune response in the survivors of the first challenge. PBMCs isolated 5 days prior to rechallenge were added to 3 peptide pools spanning the entire EBOV GP in either an IFN-γ ICS assay, or a 6 day CFSE proliferation assay. The results displayed are the average of the response to the 3 pools combined after the media only background was subtracted for each pool. (A) IFNγ production in CD4+ T cells. (B) IFNγ production in CD8+ T cells. (C) Proliferation of CD4+ T cells. (D) Proliferation of CD8+ T cells.