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Validation by Experimental Evidences: A) FAD binding site at the N-terminus, S-Adenosylmethione-binding site in the regulatory domain, tryptic cleavage, and a conserved domain is shown in the wild type MTHFR; B) MTHFR sequence showing the FAD binding site in yellow color, Adomet binding site in the pink color, tryptic cleavage site in the red color and the conserved domain in the green color.
