Figure 2.

All OPs in the Spinal Cord and Optic Nerve Are Proliferating at P21 and P60

(A–F) Dividing OPs were labeled by continuous EdU administration to P21 and P60 mice via their drinking water at 0.2 mg/ml. Images are shown of spinal cord white matter (spinal wm) labeled with Pdgfra and EdU after EdU administration from P21 (A) or P60 (B and C) and of spinal cord gray matter (spinal gm) that was EdU labeled from P21 (D) or P60 (E and F).

(G–J) The percentages of Pdgfra⁺ OPs that were EdU labeled (labeling index) were plotted against EdU labeling time (G). Also shown are images of optic nerve labeled to detect Pdgfra and EdU following labeling from P21 (H) or P60 (I and J).

(K) The EdU labeling index in optic nerve was plotted against EdU labeling time (means ±SD, n = 3 mice). The maximum labeling index (GF) was 100%. Arrows and arrowheads indicate Pdgfra⁺, EdU⁺ and Pdgfra⁺, and EdU-negative cells, respectively. Cell nuclei were stained with Hoechst 33258 (blue). Scale bars represent 25 μm.