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. Author manuscript; available in PMC: 2013 Nov 28.
Published in final edited form as: Int J Cancer. 2008 Jun 1;122(11):10.1002/ijc.23431. doi: 10.1002/ijc.23431

Figure 3.

Figure 3

PC3/BMS cell interaction induces MMP-12 expression. PC3 and BMS cells were cultured alone (a) for 24 hr on top of type I collagen-coated dishes, and the expression of various MMPs was examined by immunoblot. The amount of protein in each cell lysate was 25 μg. In another experiment, lysates (50 μg total protein) obtained from PC3 and BMS cells cultured together (PC3/BMS coculture), or separately (PC3+BMS) and combined in equal parts (25 μg total protein each), were compared (b). GADPH loading controls are shown in the lower panel of each Western blot.