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. Author manuscript; available in PMC: 2013 Nov 28.
Published in final edited form as: Int J Cancer. 2008 Jun 1;122(11):10.1002/ijc.23431. doi: 10.1002/ijc.23431

Figure 4.

Figure 4

Downregulation of MMP-12 abrogates chemoinvasion induced by PC3/BMS interaction. MMP-12 mRNA in scrambled- and MMP-12-siRNA-transfected PC3 cells, as shown by RT-PCR (a). Western blot bands detected by the antibody against MMP-12 were quantitated by densitometry. Values obtained were normalized using the housekeeping protein GAPDH, and then the ratio between the results for PC3 cells treated with MMP-12-siRNA and scrambled-siRNA (control) was calculated (b). Invasiveness of PC3 cells transiently transfected with either scrambled- or MMP-12-siRNA cultured alone or together with BMS cells (c). Representative micrographs showing invasive capacity of PC3 cells treated with scrambled- or MMP-12-siRNA and combined with BMS cells (d). Bars, 100 μm. Each experiment was performed in triplicate, and results are expressed as mean ±SE percentage of the control (PC3 + scb-siRNA). *p < 0.003 (Student’s t-test) and **p < 0.001 (Tukey–Kramer post hoc applied to significant effect of group ANOVA). [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]