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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Clin Gastroenterol. 2013 Nov-Dec;47(10):10.1097/MCG.0b013e3182a89fc8. doi: 10.1097/MCG.0b013e3182a89fc8

Figure 4.

Figure 4

Immunohistochemistry for enteroendocrine cell (EE) markers in duodenal biopsies from patients with PC1/3 deficiency compared to age matched controls.

(A) Immunostaining for chromogranin A (CGA) in control showing immunoreactive EE cell (dark brown) scattered within epithelium of crypts and villi;(B) the same immunostain as in A in patient with PC 1/3 deficiency shows similar number and distribution of CGA immunoreactive cells (immunostaning for CGA ,magnification bar for A&B, 100 microns).

(C) Immunostaning for PC1 in control showing numerous PC1 immunoreactive cells (dark brown) distributed within crypt and villous epithelium; (D) in patient with PC1/3 deficiency only very occasional PC1 immunoreactive cells (arrow) are present. (Immunostaning for PC 1, magnification bar for C&D, 50 microns).

(E) Immunostaning for PC2 in control biopsy shows scattered positive cells (arrows) within villous epithelium. (F) only a single PC2 positive cell (arrow) is seen in duodenal mucosa of PC1/3 deficient patient. (Immunostaning for PC2, magnification bar same as in Fig C–D).

(G) Immunostaning for GLP1 in control with several immunoreactive cells within villous epithelium (arrows);(H) very occasional GLP1 immunoreactive cells (arrows) are noted in patient with PC1/3 deficiency. (Immunostaning for GLP 1, magnification bar same as Fig C–D).