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. 2013 Nov 15;10:136. doi: 10.1186/1742-4690-10-136

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Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HIV-1 particles produced in the presence of stapled peptides show defects in infectivity. Six hours posttransfection with pNL4-3, 293T cells were treated with indicated concentrations of NYAD-36, -66, and -67 and virions were collected after 2 days. RT-normalized virus stocks were used to infect TZM-bl cells for 2 h in a total volume of 100 μl (the concentration of peptides from producer cells was diluted ~20-fold). Two days after infection, cells were washed, lysed and assayed for luciferase activity. N = 8, ± SD.