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. 2013 Nov 15;12:419. doi: 10.1186/1475-2875-12-419

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Copyright © 2013 Mara et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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msp2 genotyping by PCR-RFLP. HinfI restriction digest pattern of (A) FC-27 type alleles and (B) 3D7-type alleles. The sizes of fixed fragments are indicated, repetitive elements are indicated as grey boxes with sizes in bp. (C) Typical polyacrylamide gel electrophoresis result. Lane 2 contains a mixture of one FC27-type allele (WOS12) and two 3D7-type allele (330 and 370). Lanes 3 and 4 contain a single msp2 allele. (D) ImageJ fragment size analysis of the gel displayed in panel C. The 3D7 108 bp fragment and the FC27 115 bp fragment co-migrate as a single peak in case of mixed sample (lane 2).