Table 1.
Assay attributes captured during annotation
| Attribute class | Attribute | Example | Source DB |
|---|---|---|---|
| Receptor | Receptor identification: Gene symbol | OPRM1 (opioid receptor, mu 1) | EntrezGene |
| Receptor identification: Protein ID | P41597 | UniProt | |
| Receptor identification: Taxonomy ID | 9606 | NCBI | |
| GPCR class | A, B, C | IUPHAR | |
| GPCR name | Mu opioid receptor | IUPHAR | |
| Previous and unofficial names | µ, OP3, MOP, MOR, OPRM | IUPHAR | |
| Receptor type | Peptidergic | — | |
| Signal transmission | Protein Kinase A signaling cascade | Reactome | |
| Signaling pathway | Primary (or major) | IUPHAR | |
| Signaling pathway effector | Adenylate cyclase | IUPHAR | |
| Signaling pathway transducer | G alpha i/o | IUPHAR | |
| Cellular component | Eukaryotic plasma membrane | EntrezGene, GO | |
| Tissue | CNS (in rat, by ICH) | IUPHAR | |
| Physiological function | Addiction | GO | |
| Oligomer components | OPRM1, DRD1 | GPCR-OKB | |
| Oligomer's physiological relevance | Affects ligand binding | GPCR-OKB | |
| Receptor species | Human (Hs), Rat (R) | IUPHAR | |
| Biological Ligand | Ligand Name | Naloxone | DrugBank |
| Ligand function: Mechanism of action | Competitive opioid antagonist | DrugBank | |
| Ligand efficacy | Antagonist | IUPHAR | |
| Ligand chemical type | Amine | — | |
| Ligand endpoint type | IC50 | PubChem | |
| Ligand affinity value | 7.5 | IUPHAR | |
| Ligand affinity unit | nM | UO | |
| Ligand indication | Promiscuous | IUPHAR |
Note: CNS, central nervous system; ICH, immunohistochemistry; DRD1, dopamine receptor D1; IC50, concentration of an inhibitor that produces 50% inhibitory response; nM, nanomolar; UO, unit ontology.