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. 2013 Nov 15;13:115. doi: 10.1186/1475-2867-13-115

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Copyright © 2013 Wu et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Knockdown of CELF1 gene in lung cancer cells. (A, B) Infection efficiency of lentivirus containing CELF1 siRNA in A549 (A) and H1299 (B) cells. The efficiency was analyzed by fluorescence microscopic analysis of infected cells. (C, D) The comparative gene expression levels of CELF1 in normal cells, CELF1-silenced cells and non-silencing lentivirus infected cells analyzed by real time PCR. (E, F). The comparative expression levels of CELF1 protein in normal cells, CELF1-silenced cells and non-silencing lentivirus infected cells analyzed by western blotting. The values represent the mean from three independent experiments; bars represent SD.