Figure 1.
E. coli RV308 growth dynamics during different cultivation modes used in this study. The graphs illustrate experimental data for optical density (OD600nm), DOT and glucose concentration. (A) Glucose limited fed-batch cultivation on mineral salt medium with continuous feed of glucose at constant feed rate of 10 g L-1 and indicated time point of induction (dashed line). (B) and (C) same as (A) but with a downshift of DOT by a decrease of stirrer speed (arrow) and either without (B) or with (C) the addition of extra trace elements to the fermentation medium. Stirrer downshift in (B) and (C) was performed 20 min before the start of glucose feeding to ensure glucose excess during cultivations. Samples were collected from the beginning of continuous glucose feed.