Table 1.
Data overview of IC50 speed assay and stage specificity analysis
| Compound | Assay 1 (A1): | Conclusion A1 | Assay 2 (A2): | Conclusion A2 | Concluded speed of action |
|---|---|---|---|---|---|
| |
IC50 speed assay |
|
Stage specificity analysis |
|
|
| |
(ratio of IC50 24 h/IC50 72 h)a |
|
(at 6-100x IC50) |
|
|
| Chloroquine |
1.1 |
Fast |
>95% action on Rb |
Fast (cidal) |
Fast (cidal) |
| |
|
|
>95% action on Sb |
|
|
| Artesunate |
1.1 |
Fast |
>95% action on R |
Fast (cidal) |
Fast (cidal) |
| |
|
|
>95% action on S |
|
|
| Atovaquone |
3.6 |
Not fast |
~50-70% action on R |
Not fast (cidal) |
Not fast (cidal) |
| |
|
|
~75-90% action on S |
|
|
| Pyrimethamine |
8.3 |
Not fast |
~5% action on R |
Not fast (cidal) |
Not fast (cidal) |
| |
|
|
~90% action on S |
|
|
| 1 |
4.3 |
Not fast |
~0-75% action on R |
Not fast (cidal) |
Not fast (cidal) |
| |
|
|
>95% action on S |
|
|
| 2 |
1.6 |
Fast/Not fast |
~40- >95% action on R |
Not fast (cidal) |
Not fast |
| |
|
|
>95% action on S |
|
|
| 3 |
1.2 |
Fast |
>95% action on R |
Fast (cidal) |
Fast (cidal) |
| >95% action on S |
aRatios are means from ≥ 3 independent experiments performed with P. falciparum strain NF54.
bR and S are abbreviations for the ring and schizont stages, respectively.