Table 6. The effects of pre-weaning (gestation and lactation) or post-weaning diets (Diet A or Diet B) on blood and tissue gene expression levels (relative fluorescence units) in the domestic kitten (Felis catus).
| Tissue | Gene | D-D Mean | D-W Mean | W-D Mean | W-W Mean | SEM | Pre-weaning P value2 | Pre-weaning FDR 2 | Post-weaning P value3 | Post-weaning FDR 3 | Pre*Postweaning P value | Pre*Postweaning FDR |
| Blood 4 | ||||||||||||
| FASN | 0.84 | 1.11 | 1.31 | 1.29 | 0.48 | 0.11 | 1.00 | 0.52 | 1.00 | 0.45 | 1.00 | |
| GLUT1 | 4.26 | 7.63 | 11.51 | 11.40 | 4.00 | 0.08 | 0.88 | 0.58 | 1.00 | 0.56 | 1.00 | |
| GLUT4 | 26.64 | 39.72 | 44.11 | 55.19 | 18.12 | 0.12 | 1.00 | 0.25 | 1.00 | 0.92 | 1.00 | |
| INSR | 5.31 | 8.05 | 8.97 | 10.21 | 3.39 | 0.03 | 0.33 | 0.13 | 1.00 | 0.56 | 1.00 | |
| IRS-1 | 2.96 | 1.74 | 3.11 | 3.51 | 1.21 | 0.16 | 1.00 | 0.54 | 1.00 | 0.24 | 1.00 | |
| IRS-2 | 0.28 | 0.16 | 0.33 | 0.29 | 0.12 | 0.26 | 1.00 | 0.32 | 1.00 | 0.67 | 1.00 | |
| LEPT | 13.12 | 21.24 | 29.48 | 33.96 | 10.96 | 0.03 | 0.33 | 0.32 | 1.00 | 0.77 | 1.00 | |
| LIPE | 2.56 | 2.86 | 2.88 | 2.16 | 1.15 | 0.85 | 1.00 | 0.83 | 1.00 | 0.61 | 1.00 | |
| PAI-1 | 0.14 | 0.16 | 0.44 | 0.36 | 0.12 | <0.001 | 0.01 | 0.60 | 1.00 | 0.41 | 1.00 | |
| PPARG | 3.19 | 4.97 | 5.32 | 4.79 | 1.90 | 0.28 | 1.00 | 0.48 | 1.00 | 0.20 | 1.00 | |
| UCP2 | 7.44 | 10.45 | 6.45 | 7.34 | 3.11 | 0.27 | 1.00 | 0.29 | 1.00 | 0.56 | 1.00 | |
| Abdominal fat5 | ||||||||||||
| FASN | 4.60 | 4.31 | 1.59 | 4.27 | 1.90 | 0.29 | 1.00 | 0.37 | 1.00 | 0.31 | 1.00 | |
| GLUT1 | 0.55 | 0.62 | 0.86 | 0.64 | 0.32 | 0.61 | 1.00 | 0.81 | 1.00 | 0.67 | 1.00 | |
| GLUT4 | 17.09 | 20.17 | 6.96 | 17.37 | 7.29 | 0.16 | 1.00 | 0.12 | 1.00 | 0.40 | 1.00 | |
| INSR | 2.35 | 2.69 | 2.17 | 2.24 | 1.12 | 0.58 | 1.00 | 0.72 | 1.00 | 0.80 | 1.00 | |
| IRS-1 | 0.62 | 0.42 | 0.25 | 0.32 | 0.17 | 0.05 | 0.55 | 0.59 | 1.00 | 0.25 | 1.00 | |
| IRS-2 | 0.28 | 0.35 | 0.20 | 0.36 | 0.14 | 0.75 | 1.00 | 0.19 | 1.00 | 0.62 | 1.00 | |
| LEPT | 19.76 | 27.86 | 14.07 | 23.47 | 10.31 | 0.54 | 1.00 | 0.24 | 1.00 | 0.93 | 1.00 | |
| LIPE | 2.55 | 3.29 | 39.36 | 2.86 | 6.08 | 0.39 | 1.00 | 0.37 | 1.00 | 0.38 | 1.00 | |
| PAI-1 | 0.26 | 0.57 | 0.25 | 0.60 | 0.20 | 0.86 | 1.00 | 0.11 | 1.00 | 0.93 | 1.00 | |
| PPARG | 5.35 | 4.51 | 2.31 | 4.94 | 2.09 | 0.38 | 1.00 | 0.51 | 1.00 | 0.27 | 1.00 | |
| UCP2 | 1.83 | 2.23 | 1.37 | 1.55 | 0.85 | 0.29 | 1.00 | 0.57 | 1.00 | 0.83 | 1.00 | |
| Reproductive organs 6 | ||||||||||||
| FASN | 0.66 | 0.67 | 1.74 | 0.41 | 0.37 | 0.54 | 1.00 | 0.33 | 1.00 | 0.32 | 1.00 | |
| GLUT1 | 0.15 | 0.16 | 0.28 | 0.11 | 0.07 | 0.60 | 1.00 | 0.32 | 1.00 | 0.30 | 1.00 | |
| GLUT4 | 2.77 | 2.93 | 20.60 | 1.89 | 3.07 | 0.40 | 1.00 | 0.35 | 1.00 | 0.34 | 1.00 | |
| INSR | 5.90 | 3.37 | 2.33 | 4.02 | 1.95 | 0.45 | 1.00 | 0.83 | 1.00 | 0.28 | 1.00 | |
| IRS-1 | 0.67 | 0.73 | 1.04 | 0.85 | 0.33 | 0.46 | 1.00 | 0.85 | 1.00 | 0.72 | 1.00 | |
| IRS-2 | 0.30 | 0.80 | 0.75 | 0.49 | 0.25 | 0.83 | 1.00 | 0.73 | 1.00 | 0.27 | 1.00 | |
| LEPT | 0.14 | 0.11 | 0.79 | 0.14 | 0.12 | 0.36 | 1.00 | 0.36 | 1.00 | 0.40 | 1.00 | |
| LIPE | 2.28 | 2.62 | 2.32 | 1.30 | 0.93 | 0.62 | 1.00 | 0.79 | 1.00 | 0.61 | 1.00 | |
| PAI-1 | 0.27 | 0.19 | 0.21 | 0.33 | 0.11 | 0.65 | 1.00 | 0.81 | 1.00 | 0.29 | 1.00 | |
| PPARG | 1.34 | 1.60 | 2.58 | 2.17 | 0.87 | 0.42 | 1.00 | 0.94 | 1.00 | 0.77 | 1.00 | |
| UCP2 | 3.26 | 3.31 | 2.42 | 2.42 | 1.11 | 0.36 | 1.00 | 0.98 | 1.00 | 0.97 | 1.00 |
Diet A-Diet A (A-A) n = 3 females, n = 2 males.
Diet A- Diet B (A-B) n = 3 females, n = 2 males.
Diet B- Diet A (B-A) n = 3 females, n = 2 males.
Diet B- Diet B (B-B) n = 4 females, n = 1 male.
Fatty acid synthase (FASN), Glucose transporter 1 (Glut1) and 4 (Glut4), insulin receptor (INSR), insulin receptor substrate 1 (IRS1) and 2 (IRS2), Leptin (LEPT), Hormone sensitive lipase (LIPE), plasminogen activator inhibitor-1 (PAL1), Uncoupling protein 2 (UCP2), peroxisome proliferative activated receptor-γ (PPARG).
Comparisons between Diets A-A and A-B vs B-A and B-B.
Comparison between Diets A-A and B-A and B-B and A-B.
n = 5 per treatment.
n = 4 per treatment.
Ovarian or testicular tissue.
Results are presented as means ± SEM. P indicates ANOVA significance of rank transformed data and FDR indicates multiple testing adjusted P value. No effect of gender was observed.