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. 2013 Nov 28;8(11):e81551. doi: 10.1371/journal.pone.0081551

Figure 4. Involvement of serine/threonine kinase type I receptors.

Figure 4

siRNA transfection for each type I receptor gene was performed when cells were 50% to 80% confluent. 24 h later GCs were exposed (▪) or not (□) to 8 nM AMH during another 24 h. The effect of siRNA on target gene expression was determined by real time PCR (A–C, n = 4). Data were analyzed using ANOVA followed by Tukey test for all-pair comparisons. The effect of Acvr1, Bmpr1a and Bmpr1b knockdown on AMH sensitivity was analysed by Western blot using a phospho-Smad1/5/8 antibody (D, n = 4) and was quantified and normalized (E). The effect of Acvr1, Bmpr1a and Bmpr1b knockdown on Id3 expression was analyzed by real-time PCR (F, n = 3). Data were analyzed using paired t-test. * p<0.05, ** p<0.01, *** p<0.001. Only GCs transfected with siRNA against Bmpr1a present a significant decrease of AMH response.