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. 2013 Nov 28;8(11):e81551. doi: 10.1371/journal.pone.0081551

Figure 5. Granulosa cells from Bmpr1a cKO mice do not respond to AMH.

Figure 5

Amhr2-Cre; Acvr1 +/− or Amhr2-Cre; Bmpr1a +/− males were bred to Acvr1 fx/fx or Bmpr1a fx/fx females to generate females that were conditionally null for Acvr1 (A, n = 3) or Bmpr1a (B, n = 7) in GCs. GCs were exposed (▪) or not (□) to 8 nM AMH. The AMH response was tested in GCs from these cKO mice by Western blot using a phospho-Smad1/5/8 antibody (A,C). Western blots were quantified and normalized to actin levels (B n = 3, D n = 7). AMH induced the phosphorylation of Smad1/5/8 in GCs from Acvr1 cKO (A, B) mice but not in those from Bmpr1a cKO mice (C, D). Data were analyzed using paired t-test. * p<0.05, a: p = 0.058. Only Bmpr1a conditional mutant GCs present a significant decrease of AMH response.