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. 2013 Nov 28;8(11):e81551. doi: 10.1371/journal.pone.0081551

Figure 7. Smad1 and 5 are the main Smads activated by AMH in granulosa cells.

Figure 7

GCs were co-transfected with a luciferase reporter construct (UAS-luc) and different expression constructs (Smad-Gal4). Four different expression constructs were transfected in combination with the reporter construct: Smad1-Gal4, Smad5-Gal4, Smad8-Gal4 and Gal4 as a control. Cells were stimulated (▪) or not (□) with 8 nM AMH. In the absence of AMH, the fusion protein Smad-Gal4 remained in the cytoplasm which is reflected by a basal expression level of luciferase. After 24h of treatment with AMH, the Smad-Gal4 protein was phosphorylated and could translocate into the nucleus and increase luciferase expression. Firefly luciferase activity measured in control medium was set to 100 arbitrary units. The results are expressed as a percentage of stimulation of Firefly luciferase activity measured in the presence of AMH (n = 3). Data were analyzed using paired t-test. ** p<0.01.