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. 2013 Nov 28;8(11):e81945. doi: 10.1371/journal.pone.0081945

Figure 2. Xanthatin suppresses STAT3 and GSK3β signaling of human NSCLCs in a dose- and time-dependent manner.

Figure 2

(A) NSCLCs (A549, H1975, HCC827, H1650 cells) were treated with indicated concentrations of xanthatin (1, 5, 10, 20, 40 μM) for 6 h and then were subjected to Western blot for measuring protein levels of phosphor-GSK3β (Ser9), t-GSK3, phosphor-STAT3 (Tyr705) and STAT3 respectively. (B) A549 and H1975 cells were treated with 20 μM xanthatin for various time (0-12 h) and then were subjected to Western blot for measuring protein levels of phosphor-GSK3β (Ser9), GSK-3, phosphor-STAT3 (Tyr705) and STAT3 respectively.