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. 2013 Dec 1;24(23):3651–3662. doi: 10.1091/mbc.E13-05-0277

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© 2013 Seldin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — NuMA recruits dynein/dynactin to the cell cortex of keratinocytes. (A–D) Immunofluorescence analysis of endogenous NuMA, p150^glued, and DIC localization in wild-type and NuMA-knockdown mouse keratinocytes, as indicated. (E) Quantitation of cells with cortical NuMA, p150-glued, and DIC localization. n = 50 cells for each, p < 0.0001 for each. (F–I) Immunofluorescence analysis of endogenous NuMA and DIC localization in untransfected and dynamitin-GFP–transfected cells. (J) Quantitation of cells with cortical NuMA and DIC localization. n = 25 cells for each, p = 1 for NuMA, p < 0.0001 for DIC. Scale bars, 10 μm.