Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 1;24(23):3651–3662. doi: 10.1091/mbc.E13-05-0277

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Seldin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 3: — 4.1/NuMA interactions are required for cortical NuMA stability and mitotic spindle orientation. (A, B) Full-length NuMA-GFP and NuMAΔ4.1 BD-GFP constructs were transfected into wild-type cells, and immunofluorescence analysis was performed to determine localization. (C) Quantitation of cortical accumulation of GFP-tagged NuMA and NuMAΔ4.1 BD. n = 25 cells for each, p = 0.7. (D) Dot plot showing the distribution of mobile fractions of cortical and spindle pole NuMA-GFP for both WT and NuMAΔ4.1 BD. p (cortical) = 0.01; p (spindle poles) = 0.63. (E, F) FRAP analysis of full-length NuMA-GFP (FL) vs. a GFP-tagged NuMA construct lacking the 4.1-binding domain (Δ4.1 BD) transfected into wild-type cells. Recovery profiles of bleached GFP cortical signal (E) and spindle pole signal (F) from both constructs over 2 min. (G, H) Analysis of mitotic spindle alignment with the center of the cortical NuMA-GFP crescent in FL and NuMAΔ4.1 BD-GFP–transfected wild-type cells plated on 100 μM laminin. Radial histograms illustrate the distribution of spindle angles from both constructs. Histograms are grouped into 15° bins, with 0° representing a spindle aligned with the center of the NuMA crescent. n = 62 for NuMA-GFP, n = 56 for NuMAΔ4.1 BD-GFP; p = 0.004 using a Kolmogorov–Smirnov test. Scale bars, 10 μm.