FIGURE 4:

GFP-Rab11 traffic to the midbody/intercellular bridge is decreased in cells expressing Sx16-ΔTM. HeLa cells were transfected with plasmids driving overexpression of GFP-Rab11, lum-GFP, or GFP-FIP3 as indicted. At 6 h after transfection, cells were infected with adenovirus to overexpress Sx12-ΔTM or Sx16-ΔTM, and images were collected 48 h after infection. Representative images of cells in telophase, typical of three independent repeats. Parallel coverslips were fixed and stained using anti–Aurora B or anti-MKLP1, and representative images are shown.