FIGURE 7:

Sx16 and Exocyst components localize to vesicles of similar density. (A) HeLa cells in telophase were harvested, and membranes in a postnuclear supernatant were fractionated on the basis of density using iodixanol gradient analysis. Representative immunoblots of the indicated proteins. Denser membranes are at the bottom of the tube. The experiment was repeated twice more, with qualitatively similar results. Note that both Rab11-positive and Rab35-positive membranes were differentially localized to those containing Sx16. (B) HeLa cells were infected with Sx16-ΔTM or Sx12-ΔTM virus as described and immunostained with anti-Sx16 (a, d, g, and j; green) or anti-Rab11 (b, e; red) or anti-sec6 or anti-sec10 (h, k respectively; red). Merged images are shown in c, f, i, and j. Data from a typical experiment.