TABLE 1:
Yeast strains used in this study.
| Name | Relevant genotypea | Source |
|---|---|---|
| PPY1716 | MATa bar1 | Zimmerman and Kellogg (2001) |
| PPY1748 | MATa bar1 STE5-8A | Strickfaden et al. (2007) |
| PPY1777 | MATa bar1 far1∆::ADE2 | Strickfaden et al. (2007) |
| PPY1789 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 | Strickfaden et al. (2007) |
| PPY1867 | MATa bar1 cln2∆::kanMX6 | This study |
| PPY1913 | MATa bar1 HIS3::PGPD1-CLB5 | Strickfaden et al. (2007) |
| PPY1918 | MATa bar1 STE5-8A HIS3::PGPD1-CLB5 | Strickfaden et al. (2007) |
| PPY2013 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 PGAL1-CDC20::URA3Kl | This study |
| PPY2014 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 PGAL1-CDC20::URA3Kl | This study |
| PPY2019 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 mbp1∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| PPY2020 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 mbp1∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| PPY2043 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 sic1∆::TRP1Cg | This study |
| PPY2063 | MATa bar1 PGAL1-CDC20::URA3Kl | This study |
| PPY2064 | MATa bar1 PGAL1-CDC20::URA3Kl | This study |
| PPY2068 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 sic1∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| PPY2069 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 sic1∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| PPY2082 | MATa bar1 far1∆::ADE2 PGAL1-CDC20::URA3Kl | This study |
| PPY2083 | MATa bar1 far1∆::ADE2 PGAL1-CDC20::URA3Kl | This study |
| PPY2085 | MATa bar1 sic1∆::TRP1Cg | This study |
| PPY2087 | MATa bar1 far1∆::ADE2 sic1∆::TRP1Cg | This study |
| PPY2090 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 mbp1∆::TRP1Cg whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| PPY2091 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 mbp1∆::TRP1Cg whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| PPY2128 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 mbp1∆::TRP1Cg whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP137 | MATa bar1 cln2∆::kanMX6 stb1∆::natMX6 PGAL1-CDC20::URA3Kl | This study |
| YPAP138 | MATa bar1 cln2∆::kanMX6 stb1∆::natMX6 PGAL1-CDC20::URA3Kl | This study |
| YPAP141 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 stb1∆::natMX6 PGAL1-CDC20::URA3Kl | This study |
| YPAP142 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 stb1∆::natMX6 PGAL1-CDC20::URA3Kl | This study |
| YPAP143 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 rpd3∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP144 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 rpd3∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP151 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP152 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP153 | MATa bar1 cln2∆::kanMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP156 | MATa bar1 cln2∆::kanMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP157 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 stb1∆::natMX6 whi5∆::HIS3Sk | This study |
| YPAP161 | MATa bar1 cln2∆::kanMX6 stb1∆::natMX6 whi5∆::HIS3Sk | This study |
| YPAP165 | MATa bar1 cln2∆::kanMX6 PGAL1-CDC20::URA3Kl | This study |
| YPAP166 | MATa bar1 cln2∆::kanMX6 PGAL1-CDC20::URA3Kl | This study |
| YPAP167 | MATa bar1 cln2∆::kanMX6 stb1∆::natMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP168 | MATa bar1 cln2∆::kanMX6 stb1∆::natMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP171 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 stb1∆::natMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP172 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 stb1∆::natMX6 whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP203 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 rpd3∆::HIS3Sk whi5∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| YPAP204 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 rpd3∆::HIS3Sk whi5∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| YPAP208 | MATa bar1 cln2∆::natMX6 sic1∆::TRP1Cg | This study |
| YPAP209 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 sic1∆::TRP1Cg whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP210 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 sic1∆::TRP1Cg whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP236 | MATa bar1 cln2∆::natMX6 sic1∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| YPAP237 | MATa bar1 cln2∆::natMX6 sic1∆::TRP1Cg PGAL1-CDC20::URA3Kl | This study |
| YPAP238 | MATa bar1 cln2∆::natMX6 sic1∆::TRP1Cg whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP239 | MATa bar1 cln2∆::natMX6 sic1∆::TRP1Cg whi5∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP240 | MATa bar1 cln2∆::kanMX6 cdh1∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP241 | MATa bar1 cln2∆::kanMX6 cdh1∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP242 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 cdh1∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP243 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 cdh1∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP244 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 cdh1∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
| YPAP245 | MATa bar1 far1∆::ADE2 cln2∆::kanMX6 cdh1∆::HIS3Sk PGAL1-CDC20::URA3Kl | This study |
aAll strains are in the W303 background (ade2-1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1). In the PGAL1-CDC20 strains, a cassette containing the URA3Kl marker and GAL1 promoter is inserted in place of the CDC20 promoter at the native CDC20 locus.