Table 2.
Taxodione (2), 3, and analogs exhibit fungicidal effect (mg/L)*
| Yeast Strains | MIC100 | MFC* | MIC100 | MFC | MIC100 | MFC | MIC100 | MFC | MIC90 | MFC |
|---|---|---|---|---|---|---|---|---|---|---|
| 3 | 3 | 37 | 37 | 2 | 2 | 33 | 33 | Azole | Azole | |
| Cryptococcus gattii R265 | 0.4 | 0.4 | 0.8 | 0.8 | 0.8 | 0.8 | 6.4 | 12.8 | 4 | - |
| Cryptococcus neoformans H99α | 0.4 | 0.8 | 0.8 | 0.8 | 1.6 | 1.6 | 6.4 | 12.8 | 1 | 8 |
| Candida albicans SC5314 | 6.4 | 12.8 | 3.2 | 6.4 | 6.4 | >12.8 | >12.8 | >100 | 0.2 | 64 |
| Candida glabrata PAT2ISO3 | 1.6 | 6.4 | 1.6 | 3.2 | 3.2 | >6.4 | 6.4 | >12.8 | 8 | >64 |
| Candida krusei DUMC132.91 | 1.6 | 1.6 | 1.6 | 3.2 | 3.2 | 6.4 | 12.8 | >12.8 | 64 | 64 |
| Candida parapsilosis MMRL1594 | 3.2 | 6.4 | 1.6 | 3.2 | 6.4 | 12.8 | 12.8 | >100 | >64 | >64 |
| Aspergillus fumigatus Af293 | 25.6 | 25.6 | 17.1 | 17.1 | >100 | >100 | 100 | >100 | 0.4 | 12.8 |
| Aspergillus fumigatus CEA10 | 25.6 | 25.6 | 17.1 | 17.1 | >100 | >100 | 100 | >100 | 0.5 | - |
MFC is defined as the lowest drug concentration at which at least 99% of cells were killed compared to the original inocula. Suspensions from the microdilution assays after 24 h (for Candida species and Aspergillus fumigatus strains) or 48 h (for strains of Cryptococcus species) of incubation were plated on drug-free medium for obtaining the values of colony forming units (CFU) to determine the minimal fungicidal concentration (MFC). Successive 2× serial dilutions of the compounds were used. The data on the susceptibility of these strains against azole drugs (itraconazole for the mold A. fumigatus and fluconazole for all yeast species) were obtained from previous studies.13,14,15