Fig. 5.

Maturin knockdown inhibits differentiation of neural progenitors. (A–C) Whole mount in situ hybridization for tubb2b following Maturin knockdown. tubb2b expression in CoMO injected (A), uninjected (B), or MatMO2 injected embryos (C). A similar effect on tubb2b expression was also observed with MatMO1 (Fig. S1G). (D–F) Proliferation in Morpholino-injected embryos. Proliferating cells were labeled using pHH3 whole mount immunostaining in CoMO (D) and MatMO2 injected embryos (E). Insets in D and E show the 0.138 mm² area on the control and injected side of each embryo in which pHH3-positive cells were counted to generate the results (F). (G–I) Whole mount in situ hybridization for tubb2b after maturin overexpression. tubb2b expression in β-Gal (G) and maturin cRNA (H) injected embryos. In all panels of this figure, the injected side is to the right (viewer’s perspective). The s.e.m. is shown. P-values obtained using Student’s t-test: **P=0.0023. Scale bars, 400μm.