Fig. 6.

Maturin transcription is induced by, and Maturin function is required for the activity of, the proneural transcription factors. (A–D′) Anterior (A–D) and lateral (A′–D′) views of maturin expression in embryos misexpressing YFP-only (A, A′), neurog2 (B, B′), neurod1 (C, C′), and ebf3 (D, D′), respectively. Arrows show areas normally lacking maturin (A, A′), express ectopic maturin when proneural transcription factors are misexpressed (B–D′). (E–M) One blastomere of 2-cell staged embryos were injected with the indicated proneural transcription factor alone (E, H and K), with control (F, I and L) or Maturin (G, J and M) morpholinos. The effects on tubb2b expression were detected by whole mount in situ hybridization at stage 15. Right side (viewer’s perspective) is the injected side. Scale bar, 400μm.