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Cysteine racemization on IgG2λ LC. a, XIC of peptides and isotopic distribution (inset). The y axis of the inset represents relative level. Top panel, unstressed material. Bottom panel, antibody was incubated at 56 °C for 6 days at pH 9.3 in D2O. The portion of the chromatogram marked with a bracket was expanded 10 times on both panels for easier visualization. b, MS2 spectra of selected peaks shown in a.
