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. 2013 Oct 17;288(48):34364–34374. doi: 10.1074/jbc.M113.493239

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Induction of SULT1A1 and SULT1A3 by dopamine. A, concentration-dependent induction of SULT1A1/3. SK-N-MC cells were treated with dopamine (DA) for 48 h following which SULT1A1/3 protein was determined by Western blotting. B, quantitation of SULT1A1 and 1A3 mRNA by qPCR. C, concentration-dependent induction of SULT1A3 in SH-SY5Y cells treated with dopamine for 48 h. D, time-dependent induction of SULT1A1/3 by 100 μm dopamine. E, concentration-dependent increase in ERK1/2 phosphorylation following dopamine treatment for 48 h. F, time-dependent increase in ERK1/2 phosphorylation in the presence of 100 μm dopamine. All results are the mean ± S.E. (error bars), n = 3, normalized to tubulin as loading control and are expressed as percentage of control (no dopamine for A, C, and E; zero time for D and F). Asterisks indicate data significantly different (p < 0.05) from control.